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SOLVED: The M13 Reverse primer and M13 Forward (-20) primer were used to sequence (Sanger sequencing) the PCR product cloned into pCR2.1-TOPO. A segment of the sequencing chromatogram is shown in Figure
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Primer and amplicon construction. The first round of PCR uses a forward... | Download Scientific Diagram
M13-Tailed Simple Sequence Repeat (SSR) Markers in Studies of Genetic Diversity and Population Structure of Common Oat Germplasm | SpringerLink
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A tailed PCR procedure for cost‐effective, two‐order multiplex sequencing of candidate genes in polyploid plants - Gholami - 2012 - Plant Biotechnology Journal - Wiley Online Library
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